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SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype <t>Microarray</t> PM13.
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1) Product Images from "Physiological roles of an Acinetobacter -specific σ factor"

Article Title: Physiological roles of an Acinetobacter -specific σ factor

Journal: mBio

doi: 10.1128/mbio.00968-25

SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.
Figure Legend Snippet: SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.

Techniques Used: RNA Sequencing, Over Expression, Plasmid Preparation, Control, Knockdown, Two Tailed Test, Activity Assay, Fluorescence, Microarray

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Microarray:

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Article Title: Genomic and phylogenetic re-assessment of the genus Stenotrophomonas: Description of Stenotrophomonas thermophila sp. nov., and the proposal of Parastenotrophomonas gen. Nov., Pseudostenotrophomonas gen. Nov., Pedostenotrophomonas gen. Nov., and Allostenotrophomonas gen. Nov.
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RNA Sequencing:

Article Title: Plasticity in a bacterial global regulatory switch that drives a shift in antibiotic resistance and virulence
Article Snippet: Utilisation of 626 substrates was tested using phenotype microarray (PM) plates and protocols supplied by BioLog Inc, USA.

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Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
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Article Title: Physiological roles of an Acinetobacter -specific σ factor.
Article Snippet: To validate and extend these findings, we generated a CRISPR interference (CRISPRi) knockdown strain of sigAb in A. baumannii ATCC 19606 and phenotyped it in various transition metals.

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Article Title: Physiological roles of an Acinetobacter -specific σ factor
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Article Title: Genomic and phylogenetic re-assessment of the genus Stenotrophomonas: Description of Stenotrophomonas thermophila sp. nov., and the proposal of Parastenotrophomonas gen. Nov., Pseudostenotrophomonas gen. Nov., Pedostenotrophomonas gen. Nov., and Allostenotrophomonas gen. Nov.
Article Snippet: Carbon sources utilization and chemical sensitivity assays were performed using Phenotype Microarray (PM) plates PM1 and PM2A and GEN III (Biolog, Hayward, CA, USA) according to the manufacturer’s instructions.

Over Expression:

Article Title: Plasticity in a bacterial global regulatory switch that drives a shift in antibiotic resistance and virulence
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Article Title: Emerging Clostridioides difficile ribotypes have divergent metabolic phenotypes.
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Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis
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Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
Article Snippet: To address this, we exposed FLDCs to spores and employed Biolog’s Phenotype MicroArray Mammalian plates PM-M1 that are pre-loaded with an array of individual carbon nutrients known to be utilised in both glycolysis and OXPHOS metabolism ( ).

Article Title: Physiological roles of an Acinetobacter -specific σ factor.
Article Snippet: To validate and extend these findings, we generated a CRISPR interference (CRISPRi) knockdown strain of sigAb in A. baumannii ATCC 19606 and phenotyped it in various transition metals.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis .
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Article Title: Physiological roles of an Acinetobacter -specific σ factor
Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

Article Title: Genomic and phylogenetic re-assessment of the genus Stenotrophomonas: Description of Stenotrophomonas thermophila sp. nov., and the proposal of Parastenotrophomonas gen. Nov., Pseudostenotrophomonas gen. Nov., Pedostenotrophomonas gen. Nov., and Allostenotrophomonas gen. Nov.
Article Snippet: Carbon sources utilization and chemical sensitivity assays were performed using Phenotype Microarray (PM) plates PM1 and PM2A and GEN III (Biolog, Hayward, CA, USA) according to the manufacturer’s instructions.

Plasmid Preparation:

Article Title: Plasticity in a bacterial global regulatory switch that drives a shift in antibiotic resistance and virulence
Article Snippet: Utilisation of 626 substrates was tested using phenotype microarray (PM) plates and protocols supplied by BioLog Inc, USA.

Article Title: Emerging Clostridioides difficile ribotypes have divergent metabolic phenotypes.
Article Snippet: By profiling growth in Biolog Phenotype MicroArray (PM) plates, we identified a wide range of carbon sources used by the C. difficile species and compared how ribotypes differentially grew on these substrates.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activators of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts ( ).

Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
Article Snippet: To address this, we exposed FLDCs to spores and employed Biolog’s Phenotype MicroArray Mammalian plates PM-M1 that are pre-loaded with an array of individual carbon nutrients known to be utilised in both glycolysis and OXPHOS metabolism ( ).

Article Title: Physiological roles of an Acinetobacter -specific σ factor.
Article Snippet: To validate and extend these findings, we generated a CRISPR interference (CRISPRi) knockdown strain of sigAb in A. baumannii ATCC 19606 and phenotyped it in various transition metals.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis .
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activa tors of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts (Fig. 1a).

Article Title: Physiological roles of an Acinetobacter -specific σ factor
Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

Article Title: Genomic and phylogenetic re-assessment of the genus Stenotrophomonas: Description of Stenotrophomonas thermophila sp. nov., and the proposal of Parastenotrophomonas gen. Nov., Pseudostenotrophomonas gen. Nov., Pedostenotrophomonas gen. Nov., and Allostenotrophomonas gen. Nov.
Article Snippet: Carbon sources utilization and chemical sensitivity assays were performed using Phenotype Microarray (PM) plates PM1 and PM2A and GEN III (Biolog, Hayward, CA, USA) according to the manufacturer’s instructions.

Control:

Article Title: Plasticity in a bacterial global regulatory switch that drives a shift in antibiotic resistance and virulence
Article Snippet: Utilisation of 626 substrates was tested using phenotype microarray (PM) plates and protocols supplied by BioLog Inc, USA.

Article Title: Emerging Clostridioides difficile ribotypes have divergent metabolic phenotypes.
Article Snippet: By profiling growth in Biolog Phenotype MicroArray (PM) plates, we identified a wide range of carbon sources used by the C. difficile species and compared how ribotypes differentially grew on these substrates.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activators of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts ( ).

Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
Article Snippet: To address this, we exposed FLDCs to spores and employed Biolog’s Phenotype MicroArray Mammalian plates PM-M1 that are pre-loaded with an array of individual carbon nutrients known to be utilised in both glycolysis and OXPHOS metabolism ( ).

Article Title: Physiological roles of an Acinetobacter -specific σ factor.
Article Snippet: To validate and extend these findings, we generated a CRISPR interference (CRISPRi) knockdown strain of sigAb in A. baumannii ATCC 19606 and phenotyped it in various transition metals.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis .
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activa tors of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts (Fig. 1a).

Article Title: Physiological roles of an Acinetobacter -specific σ factor
Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

Article Title: Genomic and phylogenetic re-assessment of the genus Stenotrophomonas: Description of Stenotrophomonas thermophila sp. nov., and the proposal of Parastenotrophomonas gen. Nov., Pseudostenotrophomonas gen. Nov., Pedostenotrophomonas gen. Nov., and Allostenotrophomonas gen. Nov.
Article Snippet: Carbon sources utilization and chemical sensitivity assays were performed using Phenotype Microarray (PM) plates PM1 and PM2A and GEN III (Biolog, Hayward, CA, USA) according to the manufacturer’s instructions.

Two Tailed Test:

Article Title: Plasticity in a bacterial global regulatory switch that drives a shift in antibiotic resistance and virulence
Article Snippet: Utilisation of 626 substrates was tested using phenotype microarray (PM) plates and protocols supplied by BioLog Inc, USA.

Article Title: Emerging Clostridioides difficile ribotypes have divergent metabolic phenotypes.
Article Snippet: By profiling growth in Biolog Phenotype MicroArray (PM) plates, we identified a wide range of carbon sources used by the C. difficile species and compared how ribotypes differentially grew on these substrates.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activators of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts ( ).

Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
Article Snippet: To address this, we exposed FLDCs to spores and employed Biolog’s Phenotype MicroArray Mammalian plates PM-M1 that are pre-loaded with an array of individual carbon nutrients known to be utilised in both glycolysis and OXPHOS metabolism ( ).

Article Title: Physiological roles of an Acinetobacter -specific σ factor.
Article Snippet: To validate and extend these findings, we generated a CRISPR interference (CRISPRi) knockdown strain of sigAb in A. baumannii ATCC 19606 and phenotyped it in various transition metals.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis .
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activa tors of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts (Fig. 1a).

Article Title: Physiological roles of an Acinetobacter -specific σ factor
Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

Article Title: Genomic and phylogenetic re-assessment of the genus Stenotrophomonas: Description of Stenotrophomonas thermophila sp. nov., and the proposal of Parastenotrophomonas gen. Nov., Pseudostenotrophomonas gen. Nov., Pedostenotrophomonas gen. Nov., and Allostenotrophomonas gen. Nov.
Article Snippet: Carbon sources utilization and chemical sensitivity assays were performed using Phenotype Microarray (PM) plates PM1 and PM2A and GEN III (Biolog, Hayward, CA, USA) according to the manufacturer’s instructions.

Activity Assay:

Article Title: Plasticity in a bacterial global regulatory switch that drives a shift in antibiotic resistance and virulence
Article Snippet: Utilisation of 626 substrates was tested using phenotype microarray (PM) plates and protocols supplied by BioLog Inc, USA.

Article Title: Emerging Clostridioides difficile ribotypes have divergent metabolic phenotypes.
Article Snippet: By profiling growth in Biolog Phenotype MicroArray (PM) plates, we identified a wide range of carbon sources used by the C. difficile species and compared how ribotypes differentially grew on these substrates.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activators of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts ( ).

Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
Article Snippet: To address this, we exposed FLDCs to spores and employed Biolog’s Phenotype MicroArray Mammalian plates PM-M1 that are pre-loaded with an array of individual carbon nutrients known to be utilised in both glycolysis and OXPHOS metabolism ( ).

Article Title: Physiological roles of an Acinetobacter -specific σ factor.
Article Snippet: To validate and extend these findings, we generated a CRISPR interference (CRISPRi) knockdown strain of sigAb in A. baumannii ATCC 19606 and phenotyped it in various transition metals.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis .
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activa tors of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts (Fig. 1a).

Article Title: Physiological roles of an Acinetobacter -specific σ factor
Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

Article Title: Genomic and phylogenetic re-assessment of the genus Stenotrophomonas: Description of Stenotrophomonas thermophila sp. nov., and the proposal of Parastenotrophomonas gen. Nov., Pseudostenotrophomonas gen. Nov., Pedostenotrophomonas gen. Nov., and Allostenotrophomonas gen. Nov.
Article Snippet: Carbon sources utilization and chemical sensitivity assays were performed using Phenotype Microarray (PM) plates PM1 and PM2A and GEN III (Biolog, Hayward, CA, USA) according to the manufacturer’s instructions.

Fluorescence:

Article Title: Plasticity in a bacterial global regulatory switch that drives a shift in antibiotic resistance and virulence
Article Snippet: Utilisation of 626 substrates was tested using phenotype microarray (PM) plates and protocols supplied by BioLog Inc, USA.

Article Title: Emerging Clostridioides difficile ribotypes have divergent metabolic phenotypes.
Article Snippet: By profiling growth in Biolog Phenotype MicroArray (PM) plates, we identified a wide range of carbon sources used by the C. difficile species and compared how ribotypes differentially grew on these substrates.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activators of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts ( ).

Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
Article Snippet: To address this, we exposed FLDCs to spores and employed Biolog’s Phenotype MicroArray Mammalian plates PM-M1 that are pre-loaded with an array of individual carbon nutrients known to be utilised in both glycolysis and OXPHOS metabolism ( ).

Article Title: Physiological roles of an Acinetobacter -specific σ factor.
Article Snippet: To validate and extend these findings, we generated a CRISPR interference (CRISPRi) knockdown strain of sigAb in A. baumannii ATCC 19606 and phenotyped it in various transition metals.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis .
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activa tors of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts (Fig. 1a).

Article Title: Physiological roles of an Acinetobacter -specific σ factor
Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

Article Title: Genomic and phylogenetic re-assessment of the genus Stenotrophomonas: Description of Stenotrophomonas thermophila sp. nov., and the proposal of Parastenotrophomonas gen. Nov., Pseudostenotrophomonas gen. Nov., Pedostenotrophomonas gen. Nov., and Allostenotrophomonas gen. Nov.
Article Snippet: Carbon sources utilization and chemical sensitivity assays were performed using Phenotype Microarray (PM) plates PM1 and PM2A and GEN III (Biolog, Hayward, CA, USA) according to the manufacturer’s instructions.

Positron Emission Tomography:

Article Title: Plasticity in a bacterial global regulatory switch that drives a shift in antibiotic resistance and virulence
Article Snippet: Utilisation of 626 substrates was tested using phenotype microarray (PM) plates and protocols supplied by BioLog Inc, USA.

Article Title: Emerging Clostridioides difficile ribotypes have divergent metabolic phenotypes.
Article Snippet: By profiling growth in Biolog Phenotype MicroArray (PM) plates, we identified a wide range of carbon sources used by the C. difficile species and compared how ribotypes differentially grew on these substrates.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activators of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts ( ).

Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
Article Snippet: To address this, we exposed FLDCs to spores and employed Biolog’s Phenotype MicroArray Mammalian plates PM-M1 that are pre-loaded with an array of individual carbon nutrients known to be utilised in both glycolysis and OXPHOS metabolism ( ).

Article Title: Physiological roles of an Acinetobacter -specific σ factor.
Article Snippet: To validate and extend these findings, we generated a CRISPR interference (CRISPRi) knockdown strain of sigAb in A. baumannii ATCC 19606 and phenotyped it in various transition metals.

Article Title: Eco-microbiology: discovering biochemical enhancers of PET biodegradation by Piscinibacter sakaiensis .
Article Snippet: Further hypothesizing that P. sakaiensis can be readily screened for novel activa tors of PET biodegradation, we used Biolog phenotype microarray (PM) plates to search chemical/nutrient space for conditions stimulating PET-dependent bioactivity, with bacterial growth and metabolism as readouts (Fig. 1a).

Article Title: Physiological roles of an Acinetobacter -specific σ factor
Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

Article Title: Genomic and phylogenetic re-assessment of the genus Stenotrophomonas: Description of Stenotrophomonas thermophila sp. nov., and the proposal of Parastenotrophomonas gen. Nov., Pseudostenotrophomonas gen. Nov., Pedostenotrophomonas gen. Nov., and Allostenotrophomonas gen. Nov.
Article Snippet: Carbon sources utilization and chemical sensitivity assays were performed using Phenotype Microarray (PM) plates PM1 and PM2A and GEN III (Biolog, Hayward, CA, USA) according to the manufacturer’s instructions.



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Biolog Inc biolog phenotype microarray (pm) plates
SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype <t>Microarray</t> PM13.
Biolog Phenotype Microarray (Pm) Plates, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biolog phenotype microarray (pm) plates - by Bioz Stars, 2026-09
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Biolog Inc biolog phenotype mammalian microarray (pm-m) plates
SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype <t>Microarray</t> PM13.
Biolog Phenotype Mammalian Microarray (Pm M) Plates, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype <t>Microarray</t> PM13.
Phenotype Microarrays (Pms) Biolog Plates, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype <t>Microarray</t> PM13.
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SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype <t>Microarray</t> PM13.
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Biolog Inc biolog phenotype microarray pm 1–10 plates
Data for biolog phenotype <t>microarray</t> PM 1–10 plates of the pathogen Corynespora cassiicola (Utilization of the isolates of C. cassiicola from tobacco was indicated by green areas in the growth curve for each substrate).
Biolog Phenotype Microarray Pm 1–10 Plates, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Data for biolog phenotype <t>microarray</t> PM 1–10 plates of the pathogen Corynespora cassiicola (Utilization of the isolates of C. cassiicola from tobacco was indicated by green areas in the growth curve for each substrate).
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Image Search Results


SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.

Journal: mBio

Article Title: Physiological roles of an Acinetobacter -specific σ factor

doi: 10.1128/mbio.00968-25

Figure Lengend Snippet: SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.

Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

Techniques: RNA Sequencing, Over Expression, Plasmid Preparation, Control, Knockdown, Two Tailed Test, Activity Assay, Fluorescence, Microarray

Data for biolog phenotype microarray PM 1–10 plates of the pathogen Corynespora cassiicola (Utilization of the isolates of C. cassiicola from tobacco was indicated by green areas in the growth curve for each substrate).

Journal: Scientific Reports

Article Title: Characteristics of Corynespora cassiicola , the causal agent of tobacco Corynespora leaf spot, revealed by genomic and metabolic phenomic analysis

doi: 10.1038/s41598-024-67510-y

Figure Lengend Snippet: Data for biolog phenotype microarray PM 1–10 plates of the pathogen Corynespora cassiicola (Utilization of the isolates of C. cassiicola from tobacco was indicated by green areas in the growth curve for each substrate).

Article Snippet: Figure 3 Data for biolog phenotype microarray PM 1–10 plates of the pathogen Corynespora cassiicola (Utilization of the isolates of C. cassiicola from tobacco was indicated by green areas in the growth curve for each substrate).

Techniques: Microarray